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3175031d  (fluidigm)


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    Structured Review

    fluidigm 3175031d
    3175031d, supplied by fluidigm, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n7+548/Anti-pS6+%5BS235%2FS236%5D+(N7-548)-175Lu/10__1172_slash_jci198632-804-103-109
    Average 91 stars, based on 1 article reviews
    3175031d - by Bioz Stars, 2026-09
    91/100 stars

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    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pS6 [S235/S236] (175Lu) clone N7-548 , Fluidigm , 3175009A.

    Article Title: Acute Effects of Growth Hormone on the Cellular Immunologic Landscape in Pediatric Patients
    Article Snippet: pS6 , 175Lu , Fluidigm , N7-548 , 3175009A.

    Article Title: LINC01871 is exclusively expressed in T and NK cells and is highly induced upon CD4 + T cell activation
    Article Snippet: anti-pS6 -175Lu, [S235/S236], clone N7-548 , Standard Biotools , Cat3175009A; RRID: AB_2811251.

    Article Title: Multiplexed imaging mass cytometry elicits p-S6 as a therapeutic and prognostic factor in diffuse large B-cell lymphoma
    Article Snippet: CD3 Polyclonal, C-Terminal 170Er Fluidigm 3170019D CD20 H1 161Dy Fluidigm 3161029D HistoneH3 D1H2 176Yb Fluidigm 3176023D Alpha-SMA 1A4 141Pr Fluidigm 3141017D CD274 E1L3N 150Nd Fluidigm 3150031D PD-1 EPR4877(2) 165Ho Fluidigm 3165039D pS6[S235/s236] N7-548 175Lu Fluidigm 3175031D pNFkB p65 [S529] K10-895.12.50 166Er Fluidigm 3166026D p53 DO-7 143Nd Fluidigm 3143026D Ki-67 B56 168Er Fluidigm 3168022D HLA-DR LN3 174Yb Fluidigm 3174025D CD366 D5D5R 154Sm Fluidigm 3154024D CD223 D2G40 153Eu Fluidigm 3153028D CD73 EPR6115 158Gd Fluidigm 3158031D CD68 KP1 159Tb Fluidigm 3159035D CD45 D9M8I 152Sm Fluidigm 3152018D CD8 D8A8Y 162Dy Fluidigm 3162035D CD4 EPR6855 156Gd Fluidigm 3156033D Caspase-3 cleaved 5A1E 172Yb Fluidigm 3172027D C-Myc p67 9E10 164Dy Fluidigm 3164025D BCL-6 K112-91 147Sm Fluidigm 3147020D BCL-2 EPR17509 146Nd Fluidigm 3146019D CD163 MAB1607-100 151Eu R&D Conjugated Vimentin MAB2105-100 160Gd R&D Conjugated adenosine A2 alpha receptor MAB9497R-100 169Tm R&D Conjugated CD37 MAB4625-100 155Gd R&D Conjugated

    Article Title: Inflammatory bone marrow signaling in pediatric acute myeloid leukemia distinguishes patients with poor outcomes
    Article Snippet: The table below provides the Label ,Target ,Clone, Cocktail ,Vendor, and Catalog Number of all antibodies used for CyTOF in comma separated format: Label, Target, Clone, Cocktail, Vendor, Catalog Number 89Y, CD45, HI30, Surface, Standard BioTools, 3089003B 141Pr, CD3, UCHT1, Surface, Standard BioTools, 3141019B 142Nd, TLR4, HTA125, Surface, Biolegend*, 312802 143Nd, CD123, 6H6, Surface, Standard BioTools, 3143014B 5 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) 144Nd, FceR1, AER-37, Surface, Biolegend*, 334602 145Nd, CD16, 3G8, Surface, Standard BioTools, 3145008B 146Nd, CD8, RPA-T8, Surface, Standard BioTools, 3146001B 147Sm, CD11c, Bu15, Surface, Standard BioTools, 3147008B 148Nd, CD34, 581, Surface, Standard BioTools, 3148001B 149Sm, CD25, 2A3, Surface, Standard BioTools, 3149010B 150Nd, pSTAT5, 47, Intracellular, Standard BioTools, 3150005A 151Eu, CD14, M5E2, Surface, Standard BioTools, 3151009B 152Sm, CD66b, 80H3, Surface, Standard BioTools, 3152011B 153Eu, pSTAT1, 58D6, Intracellular, Standard BioTools, 3153003A 154Sm, CD10, HI10a, Surface, Biolegend*, 312202 155Gd, CD27, L128, Surface, Standard BioTools, 3155001B 156Gd, p38, D3F9, Intracellular, Standard BioTools, 3156002A 158Gd, pSTAT3, 4/P-Stat3, Intracellular, Standard BioTools, 3158005A 159Tb, CD68, Y1/48A, Intracellular, Standard BioTools, 3171011B 160Gd, TLR8, S16018A, Intracellular, Biolegend*, 395502 161Dy, CD20, H1, Intracellular, Standard BioTools, 3161029D 162Dy, Foxp3, PCH101, Intracellular, Standard BioTools, 3162011A 163Dy, CD33, p67.6, Surface, Biolegend*, 366602 164Dy, I$#", L35A5, Intracellular, Standard BioTools, 3164004A 165Ho, CD19, HIB19, Surface, Standard BioTools, 3165025B 166Er, pNFkBp65, K10895.12.50, Intracellular, Standard BioTools, 3166006A 167Er, CD38, HIT2, Surface, Standard BioTools, 3167001B 168Er, CD73, AD2, Surface, Standard BioTools, 3168015B 169Tm, CD45RA, HI100, Surface, Standard BioTools, 3169008B 170Er, CD117, 104D2, Surface, Biolegend*, 313202 171Yb, pERK1/2, D13.14.4E, Intracellular, Standard BioTools, 3171010A 172Yb, CD15, W6D3, Intracellular, Standard BioTools, 3172021B 173Yb, HLA-DR, L243, Surface, Standard BioTools, 3173005B 174Yb, CD4, SK3, Surface, Standard BioTools, 3174004B 175Lu, pS6, N7-548, Intracellular, Standard BioTools, 3175009A 176Yb, CD56, HCD56, Surface, Standard BioTools, 3176008B 209Bi, CD11b, ICRF44, Intracellular, Standard BioTools, 3209003B * Unlabeled purified antibodies were conjugated to metal isotopes using Maxpar X8 Antibody Labelling Kits (Standard BioTools) as per manufacturer's instructions.

    Recombinase Polymerase Amplification:

    Article Title: Depletion of central memory CD8 + T cells might impede the antitumor therapeutic effect of Mogamulizumab
    Article Snippet: CyTOF: 141Pr- EOMES, Fluidigm, WD1928, Custom made 142Nd- CD40, Fluidigm, 5C3, 3142010B 143Nd- CD45RA, Fluidigm, HI100, 3143006B 146Nd- CD8a, Fluidigm, RPA-T8, 3146001B 147Sm- CD11c, Fluidigm, Bu15, 3147008B 148Nd- CD14, Fluidigm, RMO52, 3148010B 149Sm- pLck [T505], Fluidigm, 4/LCK-Y505, Custom made 150Nd- CD134 (OX40), Fluidigm, ACT35, 3150023B 151Eu- CD107a (LAMP1), Fluidigm, H4A3, 3151002B 153Eu- CD194 (CCR4), Fluidigm, 205410, 3153013A 154Sm- CD3, Fluidigm, UCHT1, 3154003B 155Gd- CD279 (PD-1), Fluidigm, EH12.2H7, 3155009B 156Gd- CD86, Fluidigm, IT2.2, 3156008B 158Gd- pStat3 [Y705], Fluidigm, 4, 3158005A 159Tb- FoxP3, Fluidigm, 236A/E7, Custom made 160Gd- Tbet, Fluidigm, 4B10, 3160010B 161Dy- CD152 (CTLA-4), Fluidigm, 14D3, 3161004B 162Dy- CD80 (B7-1), Fluidigm, 2D10.4, 3162010B 163Dy- CD183 (CXCR3), Fluidigm, G025H7, 3163004B 164Dy- CD185 (CXCR5), Fluidigm, 51505, 3164016B 3 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Validation Eukaryotic cell lines Policy information about cell lines Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Wild animals Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. .. 165Ho- CD223 (LAG3), Fluidigm, 874501, 3165028B 166Er- pNF-kB p65 [S529], Fluidigm, K10-895.12.50, 3166006A 3 nature research | reporting summary April 2020 167Er- CD197 (CCR7), Fluidigm, G043H7, 3167009A 168Er- CD154 (CD40L), Fluidigm, 24-31, 3168006B 169Tm- CD25 (IL-2R), Fluidigm, 2A3, 3169003B 170Yb- CCR8, Fluidigm, L263G8, Custom made 171Yb- CD20, Fluidigm, 2H7, 3171012B 172Yb- pS6 [S235/S236], Fluidigm, N7-548, 3172008A 175Lu- CD274 (PD-L1), Fluidigm, 29E.2A3, 3175017B 176Yb- CD4, Fluidigm, RPA-T4, 3176010B Flow cytometry: AF-700- Human CD3, BD Biosciences, UCHT1, 561027 BV510- Human CD4, BD Biosciences, SK3, 562970 BV786- Human CD8, BD Biosciences, RPA-T8, 563823 PerCP-Cy5.5- Human CCR4, BD Biosciences, 1G1, 560726 BV711 and AF488- Human CD45RA, BioLegend, HI100, 304137 BV421- Human PD-1, BD Biosciences, MIH4, 564323 PE- Human FoxP3, eBioscience, 236A/E7, 72-5774-40 APC- Human CTLA-4, BD Biosciences, BNI3, 557301 FITC- Human LAG3, Enzo, 17B4, ALX-804-806F-C100 PE-CF594- Human CCR7, BD Biosciences, 150503, 562381 NIR- Zombie NIR, BioLegend, 423105 ADCC activity for NK cell: Unconjugated Human NKG2C, R&D systems, 134522, MAB1381-100 In vivo and vitro experiment for T cell depletion: Mogamulizumab, Kyowakirin, KW-0761 Ultra-LEAF Purified Human IgG1 Isotype control, BioLegend, QA16A12, 403501 These antibodies that are commercially available were validated by the manufacturers and used according to the manufacturer's instructions. ..

    Flow Cytometry:

    Article Title: Depletion of central memory CD8 + T cells might impede the antitumor therapeutic effect of Mogamulizumab
    Article Snippet: CyTOF: 141Pr- EOMES, Fluidigm, WD1928, Custom made 142Nd- CD40, Fluidigm, 5C3, 3142010B 143Nd- CD45RA, Fluidigm, HI100, 3143006B 146Nd- CD8a, Fluidigm, RPA-T8, 3146001B 147Sm- CD11c, Fluidigm, Bu15, 3147008B 148Nd- CD14, Fluidigm, RMO52, 3148010B 149Sm- pLck [T505], Fluidigm, 4/LCK-Y505, Custom made 150Nd- CD134 (OX40), Fluidigm, ACT35, 3150023B 151Eu- CD107a (LAMP1), Fluidigm, H4A3, 3151002B 153Eu- CD194 (CCR4), Fluidigm, 205410, 3153013A 154Sm- CD3, Fluidigm, UCHT1, 3154003B 155Gd- CD279 (PD-1), Fluidigm, EH12.2H7, 3155009B 156Gd- CD86, Fluidigm, IT2.2, 3156008B 158Gd- pStat3 [Y705], Fluidigm, 4, 3158005A 159Tb- FoxP3, Fluidigm, 236A/E7, Custom made 160Gd- Tbet, Fluidigm, 4B10, 3160010B 161Dy- CD152 (CTLA-4), Fluidigm, 14D3, 3161004B 162Dy- CD80 (B7-1), Fluidigm, 2D10.4, 3162010B 163Dy- CD183 (CXCR3), Fluidigm, G025H7, 3163004B 164Dy- CD185 (CXCR5), Fluidigm, 51505, 3164016B 3 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Validation Eukaryotic cell lines Policy information about cell lines Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Wild animals Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. .. 165Ho- CD223 (LAG3), Fluidigm, 874501, 3165028B 166Er- pNF-kB p65 [S529], Fluidigm, K10-895.12.50, 3166006A 3 nature research | reporting summary April 2020 167Er- CD197 (CCR7), Fluidigm, G043H7, 3167009A 168Er- CD154 (CD40L), Fluidigm, 24-31, 3168006B 169Tm- CD25 (IL-2R), Fluidigm, 2A3, 3169003B 170Yb- CCR8, Fluidigm, L263G8, Custom made 171Yb- CD20, Fluidigm, 2H7, 3171012B 172Yb- pS6 [S235/S236], Fluidigm, N7-548, 3172008A 175Lu- CD274 (PD-L1), Fluidigm, 29E.2A3, 3175017B 176Yb- CD4, Fluidigm, RPA-T4, 3176010B Flow cytometry: AF-700- Human CD3, BD Biosciences, UCHT1, 561027 BV510- Human CD4, BD Biosciences, SK3, 562970 BV786- Human CD8, BD Biosciences, RPA-T8, 563823 PerCP-Cy5.5- Human CCR4, BD Biosciences, 1G1, 560726 BV711 and AF488- Human CD45RA, BioLegend, HI100, 304137 BV421- Human PD-1, BD Biosciences, MIH4, 564323 PE- Human FoxP3, eBioscience, 236A/E7, 72-5774-40 APC- Human CTLA-4, BD Biosciences, BNI3, 557301 FITC- Human LAG3, Enzo, 17B4, ALX-804-806F-C100 PE-CF594- Human CCR7, BD Biosciences, 150503, 562381 NIR- Zombie NIR, BioLegend, 423105 ADCC activity for NK cell: Unconjugated Human NKG2C, R&D systems, 134522, MAB1381-100 In vivo and vitro experiment for T cell depletion: Mogamulizumab, Kyowakirin, KW-0761 Ultra-LEAF Purified Human IgG1 Isotype control, BioLegend, QA16A12, 403501 These antibodies that are commercially available were validated by the manufacturers and used according to the manufacturer's instructions. ..

    Activity Assay:

    Article Title: Depletion of central memory CD8 + T cells might impede the antitumor therapeutic effect of Mogamulizumab
    Article Snippet: CyTOF: 141Pr- EOMES, Fluidigm, WD1928, Custom made 142Nd- CD40, Fluidigm, 5C3, 3142010B 143Nd- CD45RA, Fluidigm, HI100, 3143006B 146Nd- CD8a, Fluidigm, RPA-T8, 3146001B 147Sm- CD11c, Fluidigm, Bu15, 3147008B 148Nd- CD14, Fluidigm, RMO52, 3148010B 149Sm- pLck [T505], Fluidigm, 4/LCK-Y505, Custom made 150Nd- CD134 (OX40), Fluidigm, ACT35, 3150023B 151Eu- CD107a (LAMP1), Fluidigm, H4A3, 3151002B 153Eu- CD194 (CCR4), Fluidigm, 205410, 3153013A 154Sm- CD3, Fluidigm, UCHT1, 3154003B 155Gd- CD279 (PD-1), Fluidigm, EH12.2H7, 3155009B 156Gd- CD86, Fluidigm, IT2.2, 3156008B 158Gd- pStat3 [Y705], Fluidigm, 4, 3158005A 159Tb- FoxP3, Fluidigm, 236A/E7, Custom made 160Gd- Tbet, Fluidigm, 4B10, 3160010B 161Dy- CD152 (CTLA-4), Fluidigm, 14D3, 3161004B 162Dy- CD80 (B7-1), Fluidigm, 2D10.4, 3162010B 163Dy- CD183 (CXCR3), Fluidigm, G025H7, 3163004B 164Dy- CD185 (CXCR5), Fluidigm, 51505, 3164016B 3 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Validation Eukaryotic cell lines Policy information about cell lines Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Wild animals Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. .. 165Ho- CD223 (LAG3), Fluidigm, 874501, 3165028B 166Er- pNF-kB p65 [S529], Fluidigm, K10-895.12.50, 3166006A 3 nature research | reporting summary April 2020 167Er- CD197 (CCR7), Fluidigm, G043H7, 3167009A 168Er- CD154 (CD40L), Fluidigm, 24-31, 3168006B 169Tm- CD25 (IL-2R), Fluidigm, 2A3, 3169003B 170Yb- CCR8, Fluidigm, L263G8, Custom made 171Yb- CD20, Fluidigm, 2H7, 3171012B 172Yb- pS6 [S235/S236], Fluidigm, N7-548, 3172008A 175Lu- CD274 (PD-L1), Fluidigm, 29E.2A3, 3175017B 176Yb- CD4, Fluidigm, RPA-T4, 3176010B Flow cytometry: AF-700- Human CD3, BD Biosciences, UCHT1, 561027 BV510- Human CD4, BD Biosciences, SK3, 562970 BV786- Human CD8, BD Biosciences, RPA-T8, 563823 PerCP-Cy5.5- Human CCR4, BD Biosciences, 1G1, 560726 BV711 and AF488- Human CD45RA, BioLegend, HI100, 304137 BV421- Human PD-1, BD Biosciences, MIH4, 564323 PE- Human FoxP3, eBioscience, 236A/E7, 72-5774-40 APC- Human CTLA-4, BD Biosciences, BNI3, 557301 FITC- Human LAG3, Enzo, 17B4, ALX-804-806F-C100 PE-CF594- Human CCR7, BD Biosciences, 150503, 562381 NIR- Zombie NIR, BioLegend, 423105 ADCC activity for NK cell: Unconjugated Human NKG2C, R&D systems, 134522, MAB1381-100 In vivo and vitro experiment for T cell depletion: Mogamulizumab, Kyowakirin, KW-0761 Ultra-LEAF Purified Human IgG1 Isotype control, BioLegend, QA16A12, 403501 These antibodies that are commercially available were validated by the manufacturers and used according to the manufacturer's instructions. ..

    In Vivo:

    Article Title: Depletion of central memory CD8 + T cells might impede the antitumor therapeutic effect of Mogamulizumab
    Article Snippet: CyTOF: 141Pr- EOMES, Fluidigm, WD1928, Custom made 142Nd- CD40, Fluidigm, 5C3, 3142010B 143Nd- CD45RA, Fluidigm, HI100, 3143006B 146Nd- CD8a, Fluidigm, RPA-T8, 3146001B 147Sm- CD11c, Fluidigm, Bu15, 3147008B 148Nd- CD14, Fluidigm, RMO52, 3148010B 149Sm- pLck [T505], Fluidigm, 4/LCK-Y505, Custom made 150Nd- CD134 (OX40), Fluidigm, ACT35, 3150023B 151Eu- CD107a (LAMP1), Fluidigm, H4A3, 3151002B 153Eu- CD194 (CCR4), Fluidigm, 205410, 3153013A 154Sm- CD3, Fluidigm, UCHT1, 3154003B 155Gd- CD279 (PD-1), Fluidigm, EH12.2H7, 3155009B 156Gd- CD86, Fluidigm, IT2.2, 3156008B 158Gd- pStat3 [Y705], Fluidigm, 4, 3158005A 159Tb- FoxP3, Fluidigm, 236A/E7, Custom made 160Gd- Tbet, Fluidigm, 4B10, 3160010B 161Dy- CD152 (CTLA-4), Fluidigm, 14D3, 3161004B 162Dy- CD80 (B7-1), Fluidigm, 2D10.4, 3162010B 163Dy- CD183 (CXCR3), Fluidigm, G025H7, 3163004B 164Dy- CD185 (CXCR5), Fluidigm, 51505, 3164016B 3 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Validation Eukaryotic cell lines Policy information about cell lines Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Wild animals Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. .. 165Ho- CD223 (LAG3), Fluidigm, 874501, 3165028B 166Er- pNF-kB p65 [S529], Fluidigm, K10-895.12.50, 3166006A 3 nature research | reporting summary April 2020 167Er- CD197 (CCR7), Fluidigm, G043H7, 3167009A 168Er- CD154 (CD40L), Fluidigm, 24-31, 3168006B 169Tm- CD25 (IL-2R), Fluidigm, 2A3, 3169003B 170Yb- CCR8, Fluidigm, L263G8, Custom made 171Yb- CD20, Fluidigm, 2H7, 3171012B 172Yb- pS6 [S235/S236], Fluidigm, N7-548, 3172008A 175Lu- CD274 (PD-L1), Fluidigm, 29E.2A3, 3175017B 176Yb- CD4, Fluidigm, RPA-T4, 3176010B Flow cytometry: AF-700- Human CD3, BD Biosciences, UCHT1, 561027 BV510- Human CD4, BD Biosciences, SK3, 562970 BV786- Human CD8, BD Biosciences, RPA-T8, 563823 PerCP-Cy5.5- Human CCR4, BD Biosciences, 1G1, 560726 BV711 and AF488- Human CD45RA, BioLegend, HI100, 304137 BV421- Human PD-1, BD Biosciences, MIH4, 564323 PE- Human FoxP3, eBioscience, 236A/E7, 72-5774-40 APC- Human CTLA-4, BD Biosciences, BNI3, 557301 FITC- Human LAG3, Enzo, 17B4, ALX-804-806F-C100 PE-CF594- Human CCR7, BD Biosciences, 150503, 562381 NIR- Zombie NIR, BioLegend, 423105 ADCC activity for NK cell: Unconjugated Human NKG2C, R&D systems, 134522, MAB1381-100 In vivo and vitro experiment for T cell depletion: Mogamulizumab, Kyowakirin, KW-0761 Ultra-LEAF Purified Human IgG1 Isotype control, BioLegend, QA16A12, 403501 These antibodies that are commercially available were validated by the manufacturers and used according to the manufacturer's instructions. ..

    Purification:

    Article Title: Depletion of central memory CD8 + T cells might impede the antitumor therapeutic effect of Mogamulizumab
    Article Snippet: CyTOF: 141Pr- EOMES, Fluidigm, WD1928, Custom made 142Nd- CD40, Fluidigm, 5C3, 3142010B 143Nd- CD45RA, Fluidigm, HI100, 3143006B 146Nd- CD8a, Fluidigm, RPA-T8, 3146001B 147Sm- CD11c, Fluidigm, Bu15, 3147008B 148Nd- CD14, Fluidigm, RMO52, 3148010B 149Sm- pLck [T505], Fluidigm, 4/LCK-Y505, Custom made 150Nd- CD134 (OX40), Fluidigm, ACT35, 3150023B 151Eu- CD107a (LAMP1), Fluidigm, H4A3, 3151002B 153Eu- CD194 (CCR4), Fluidigm, 205410, 3153013A 154Sm- CD3, Fluidigm, UCHT1, 3154003B 155Gd- CD279 (PD-1), Fluidigm, EH12.2H7, 3155009B 156Gd- CD86, Fluidigm, IT2.2, 3156008B 158Gd- pStat3 [Y705], Fluidigm, 4, 3158005A 159Tb- FoxP3, Fluidigm, 236A/E7, Custom made 160Gd- Tbet, Fluidigm, 4B10, 3160010B 161Dy- CD152 (CTLA-4), Fluidigm, 14D3, 3161004B 162Dy- CD80 (B7-1), Fluidigm, 2D10.4, 3162010B 163Dy- CD183 (CXCR3), Fluidigm, G025H7, 3163004B 164Dy- CD185 (CXCR5), Fluidigm, 51505, 3164016B 3 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Validation Eukaryotic cell lines Policy information about cell lines Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Wild animals Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. .. 165Ho- CD223 (LAG3), Fluidigm, 874501, 3165028B 166Er- pNF-kB p65 [S529], Fluidigm, K10-895.12.50, 3166006A 3 nature research | reporting summary April 2020 167Er- CD197 (CCR7), Fluidigm, G043H7, 3167009A 168Er- CD154 (CD40L), Fluidigm, 24-31, 3168006B 169Tm- CD25 (IL-2R), Fluidigm, 2A3, 3169003B 170Yb- CCR8, Fluidigm, L263G8, Custom made 171Yb- CD20, Fluidigm, 2H7, 3171012B 172Yb- pS6 [S235/S236], Fluidigm, N7-548, 3172008A 175Lu- CD274 (PD-L1), Fluidigm, 29E.2A3, 3175017B 176Yb- CD4, Fluidigm, RPA-T4, 3176010B Flow cytometry: AF-700- Human CD3, BD Biosciences, UCHT1, 561027 BV510- Human CD4, BD Biosciences, SK3, 562970 BV786- Human CD8, BD Biosciences, RPA-T8, 563823 PerCP-Cy5.5- Human CCR4, BD Biosciences, 1G1, 560726 BV711 and AF488- Human CD45RA, BioLegend, HI100, 304137 BV421- Human PD-1, BD Biosciences, MIH4, 564323 PE- Human FoxP3, eBioscience, 236A/E7, 72-5774-40 APC- Human CTLA-4, BD Biosciences, BNI3, 557301 FITC- Human LAG3, Enzo, 17B4, ALX-804-806F-C100 PE-CF594- Human CCR7, BD Biosciences, 150503, 562381 NIR- Zombie NIR, BioLegend, 423105 ADCC activity for NK cell: Unconjugated Human NKG2C, R&D systems, 134522, MAB1381-100 In vivo and vitro experiment for T cell depletion: Mogamulizumab, Kyowakirin, KW-0761 Ultra-LEAF Purified Human IgG1 Isotype control, BioLegend, QA16A12, 403501 These antibodies that are commercially available were validated by the manufacturers and used according to the manufacturer's instructions. ..

    Control:

    Article Title: Depletion of central memory CD8 + T cells might impede the antitumor therapeutic effect of Mogamulizumab
    Article Snippet: CyTOF: 141Pr- EOMES, Fluidigm, WD1928, Custom made 142Nd- CD40, Fluidigm, 5C3, 3142010B 143Nd- CD45RA, Fluidigm, HI100, 3143006B 146Nd- CD8a, Fluidigm, RPA-T8, 3146001B 147Sm- CD11c, Fluidigm, Bu15, 3147008B 148Nd- CD14, Fluidigm, RMO52, 3148010B 149Sm- pLck [T505], Fluidigm, 4/LCK-Y505, Custom made 150Nd- CD134 (OX40), Fluidigm, ACT35, 3150023B 151Eu- CD107a (LAMP1), Fluidigm, H4A3, 3151002B 153Eu- CD194 (CCR4), Fluidigm, 205410, 3153013A 154Sm- CD3, Fluidigm, UCHT1, 3154003B 155Gd- CD279 (PD-1), Fluidigm, EH12.2H7, 3155009B 156Gd- CD86, Fluidigm, IT2.2, 3156008B 158Gd- pStat3 [Y705], Fluidigm, 4, 3158005A 159Tb- FoxP3, Fluidigm, 236A/E7, Custom made 160Gd- Tbet, Fluidigm, 4B10, 3160010B 161Dy- CD152 (CTLA-4), Fluidigm, 14D3, 3161004B 162Dy- CD80 (B7-1), Fluidigm, 2D10.4, 3162010B 163Dy- CD183 (CXCR3), Fluidigm, G025H7, 3163004B 164Dy- CD185 (CXCR5), Fluidigm, 51505, 3164016B 3 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Validation Eukaryotic cell lines Policy information about cell lines Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Wild animals Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. .. 165Ho- CD223 (LAG3), Fluidigm, 874501, 3165028B 166Er- pNF-kB p65 [S529], Fluidigm, K10-895.12.50, 3166006A 3 nature research | reporting summary April 2020 167Er- CD197 (CCR7), Fluidigm, G043H7, 3167009A 168Er- CD154 (CD40L), Fluidigm, 24-31, 3168006B 169Tm- CD25 (IL-2R), Fluidigm, 2A3, 3169003B 170Yb- CCR8, Fluidigm, L263G8, Custom made 171Yb- CD20, Fluidigm, 2H7, 3171012B 172Yb- pS6 [S235/S236], Fluidigm, N7-548, 3172008A 175Lu- CD274 (PD-L1), Fluidigm, 29E.2A3, 3175017B 176Yb- CD4, Fluidigm, RPA-T4, 3176010B Flow cytometry: AF-700- Human CD3, BD Biosciences, UCHT1, 561027 BV510- Human CD4, BD Biosciences, SK3, 562970 BV786- Human CD8, BD Biosciences, RPA-T8, 563823 PerCP-Cy5.5- Human CCR4, BD Biosciences, 1G1, 560726 BV711 and AF488- Human CD45RA, BioLegend, HI100, 304137 BV421- Human PD-1, BD Biosciences, MIH4, 564323 PE- Human FoxP3, eBioscience, 236A/E7, 72-5774-40 APC- Human CTLA-4, BD Biosciences, BNI3, 557301 FITC- Human LAG3, Enzo, 17B4, ALX-804-806F-C100 PE-CF594- Human CCR7, BD Biosciences, 150503, 562381 NIR- Zombie NIR, BioLegend, 423105 ADCC activity for NK cell: Unconjugated Human NKG2C, R&D systems, 134522, MAB1381-100 In vivo and vitro experiment for T cell depletion: Mogamulizumab, Kyowakirin, KW-0761 Ultra-LEAF Purified Human IgG1 Isotype control, BioLegend, QA16A12, 403501 These antibodies that are commercially available were validated by the manufacturers and used according to the manufacturer's instructions. ..



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